Comparison of Two Protein Extraction Methods for Tea Residues



Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).




Honghua Tan






X. Chen et al., "Comparison of Two Protein Extraction Methods for Tea Residues", Applied Mechanics and Materials, Vols. 138-139, pp. 933-936, 2012


November 2011




[1] Kondo, M., K. Kita, and H. Yokota, Feeding value to goats of whole-crop oat ensiled with green tea waste. Animal feed science and technology, 2004. 113(1-4): pp.71-81.

DOI: 10.1016/j.anifeedsci.2003.10.018

[2] Gülser, C. and A. Peken, Using tea waste as a new casing material in mushroom (Agaricus bisporus (L. ) Sing. ) cultivation. Bioresource technology, 2003. 88(2): pp.153-156.

DOI: 10.1016/s0960-8524(02)00279-1

[3] Ahluwalia, S. and D. Goyal, Removal of heavy metals by waste tea leaves from aqueous solution. Engineering in Life Sciences, 2005. 5(2): pp.158-162.

DOI: 10.1002/elsc.200420066

[4] Abd-elfattah, A.M. and M. Abd-elbasseer, Characterization of Poly-isoprene Rubber Layer Backed with Porous Material as Sound Absorber and Vibration Damper. Journal of American Science, 2011. 7(2).

[5] Tsubaki, S., et al., Microwave heating of tea residue yields polysaccharides, polyphenols, and plant biopolyester. Journal of agricultural and food chemistry, 2008. 56(23): pp.11293-11299.

DOI: 10.1021/jf802253s

[6] Tsubaki, S., M. Sakamoto, and J. Azuma, Microwave-assisted extraction of phenolic compounds from tea residues under autohydrolytic conditions. Food Chemistry, 2010. 123(4): pp.1255-1258.

DOI: 10.1016/j.foodchem.2010.05.088

[7] Shen, L., et al., Studies on tea protein extraction using alkaline and enzyme methods. Food Chemistry, 2008. 107(2): pp.929-938.

DOI: 10.1016/j.foodchem.2007.08.047

[8] Gu, L., Lu, J., & Ye, B. Tea chemistry. Hefei: Chinese University of Science and Technology Publishing. (2002).

[9] Shen L, Huang G, Wang X, et. al. Extraction of Protein from Tea Sullage by Protease, Journal of Food Science and Biotechnology, 2006, 25(6): 7-12, 33.

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