Bio-Inspired Fabrication of Polymer Composite Scaffolds with Chitosan Network inside the Pore Channels



Solid freeform fabrication, known as rapid prototyping (RP) technology allows in designing the scaffold with pre-defined and controlled external and internal architecture.In this study we produce scaffolds with network of chitosan fibrils that mimic the extracellular matrix produced by the cells. These network scaffolds also consisting of nanoparticles of hydroxyapatite (HA) for stabilisation of scaffolds are characterised by environmental scanning electron microscopy and mechanical properties. ESEM showed that the scaffolds possess macropore (300µm), micropore and fibre network structure. The compressive strength and elastic modulus (E) for the scaffolds are 0.54± 0.02 MPa and 6.13± 0.60 MPa, respectively, which are increasing obviously. The biocompatibility of the woodpile-network scaffolds was investigated with osteoblastic cells. The result showed the distribution and proliferation of osteoblast orients along the chtosan fibre network, preferentially. After 4 weeks of culture, macropore channels are covered by cells in large part,while the areas without chitosan fibre network are covered rarely. The properties of these scaffolds indicate that they can be used for bone tissue engineering applications.



Robert Zhu




B. Li et al., "Bio-Inspired Fabrication of Polymer Composite Scaffolds with Chitosan Network inside the Pore Channels", Applied Mechanics and Materials, Vol. 140, pp. 38-42, 2012


November 2011




[1] R. Langer, J. P. Vacanti, Tissue engineering, J. Science. 260(1993) 920–926.

[2] S. Kale, S. Biermann, C. Edwards, C. Tarnowski, M. Morris, M.W. Long, Three-dimensional cellular development is essential for ex vivo formation of human bone, J. Nature Biotechnology. 18(2000) 954-958.


[3] Hutmacher DW, Polymeric Scaffolds in Tissue Engineering Bone and Cartilage, J. Biomaterials. 21(2000) 2529-2543.


[4] V. Midy, M. Dard, E.J. Hollande, Evaluation of the effect of three calcium phosphate powders on osteoblast cells, J. Journal of Materials Science: Materials in Medicine. 12(2001) 259-265.


[5] L. H. Li, C. R. Zhou, S.J. Ding, Preparation and degradation of PLA/chitosan composite materials, J. Journal of Applied Polymer Science. 91(2004) 274-277.


[6] E. Eisenbarth, Biomaterials for Tissue Engineering, J. Advanced Engineering Materials. 9(2007) 1051-1060.

[7] I. Manjubala, Alexander Woesz, Christine Pilz, Monika Rumpler, Nadja Fratzl-Zelman, Paul Roschger, Jürgen Stampfl, Peter Fratzl, Biomimetic mineral-organic composite scaffolds with controlled internal architecture, J. Journal of Materials Science: Materials in Medicine. 16(2005).


[8] K. Whang, K.E. Healy, D.R. Elenz, E.K. Nam, D.C. Tsai, C.H. Thomas, G.W. Nuber, F.H. Glorieux, R. Travers, S.M. Sprague, Engineering Bone Regeneration with Bioabsorbable Scaffolds with Novel Microarchitecture, J. Tissue Engineering. 5(1999) 53-65.


[9] X. L. Zhu, J. Chen, L. Scheideler, R. Reichl, J. Geis-Gerstorfer, Effects of topography and composition of titanium surface oxides on osteoblast responses, J. Biomaterials. 25(2004) 4087–4103.