URU International Conference on Science and Technology 2018

文章题目 页数

作者: Wutthikrai Kulsawat, Boonsom Porntepkasemsan, Phatchada Nochit
摘要: Paddy residues are the most generous agricultural biomass from the paddy cultivation, Paddy residues practices include crop residue amendment and in-situ burning. It indicated that residue amendment increased the organic carbon and nutrient contents in soil, However, an open residue burning is still a common practice in Thailand despite of strict law enforcements and proper education to farmers about its implications on soil, human and animal health The present study determined how residues management practices: residue amendment and stubble burning, influence the soil organic carbon by determining δ13C in paddy soil profile. The 30 cm depth soil samples from the naturally straw amendment and stubble burning paddy fields were collected in Chiang Khwan district, Roi-et province during 2017. The δ13C values with soil depth showed that residue management practices produce statistical differences in both soils. The δ13C values of soil samples from amendment and burning sites ranged from-23.19‰ to-17.98‰ and-24.79‰ to-19.28‰, respectively. Carbon isotopes differentiate clearly between amendment site (more positive values) and burning site (more negative values). The results from this study were in accordance with literatures which reported that the δ13C distribution in the soil profile can be applied to study in SOC dynamics as a result of different paddy residue management practices (amendment or burning). Further research is needed to confirm the validity of the stable carbon isotope technique in this type of studies.
作者: Boonsom Porntepkasemsan, Wutthikrai Kulsawat, Phatchada Nochit
摘要: Water scarcity resulting from erratic rainfall pattern is a major problem over the world. As diminishing water resources for agriculture and increasing demand for rice yield, understanding on hydrological cycle is crucial on water management. The loss of water due to evaporation from rivers and canals constitutes a substantial part of useable water. The estimated evaporation loss can be obtained by using the isotope compositions of precipitation and an established local evaporation line. It is recognized a very useful tool in providing a quick and cost effective insight into the water balance of surface water pool. In this study of 2015-2017 in Suphanburi province, the isotopic signatures of precipitation and surface water canal resulted in a local meteoric water line of δ2H = 7.63 δ18O – 1.05 (n = 168, r2 = 0.94) and local evaporation line for surface water creek of δ2H = 5.95 δ18O – 14.42 (n = 155, r2 = 0.90), respectively. The estimated evaporation loss using the Hydrocalculator software indicated that the estimated fractional water loss by evaporation in the surface water canal of Sri Prachan district, Suphanburi was ~12%.
作者: Ekawit Threenet, Achara Kleawkla, Yossaporn Kaewkalong, Winai Wiriyaalongkorn, Adisak Joomwong, Theerawee Kleawkla, Thritsanat Kleawkla
摘要: Proteomic investigation of leaves, peels and seeds on longan (Dimocarpus longan Lour. cv. Daw) at Maejo University’s farm in Chiang Mai province, Thailand. There were comparisons between normal and physiological disorder syndromes in longan on fruit growth (5, 10, 15, 20, 25 and 30 weeks fruition, respectively) by one dimensional electrophoresis (1-D gel) technique at a concentration of 12.5% gel in reducing condition with Coomassie brilliant blue R-250 staining coupling on liquid chromatography tandem mass spectrometry (LC-MS/MS). The research showed that vegetative storage protein (25.2 KDa), a storage protein in plants, was expressed in leaves, peels and seeds on before and after physiological disorder syndrome occurring. A protein involved on photosynthesis, the ribulose-1,5-bisphosphate carboxylase/oxygenase (48.3 KDa), was represented only on leaves at 30 weeks after fruition but not in peels and seeds of longan. Two proteins, the BnaC01g20210 (35.8 KDa) and predicted protein (40.11 KDa), which could not be identified the type and function of the metabolism, were decreased in diseased longan. These proteins may be important protein in part of the recovery process of abnormal longan. Therefore, advanced technique will be used for further proteomic studies.
作者: Achara Kleawkla, Ekawit Threenet, Wanlapha Khonkham, Winai Wiriyaalongkorn, Adisak Joomwong, Theerawee Kleawkla, Thritsanat Kleawkla
摘要: Three procedures for protein extraction in longan pulp had been applied to analyze protein pattern and quality of Longan pulp (Dimocarpus longan Lour. cv. Daw) during fruit growth to increase protein expression in proteomic analysis at Maejo university’s farm. There were data points to compare between normal and physiological disorder syndromes during fruit growth (5,10, 15, 20, 25 and 30 weeks, respectively) by one dimensional electrophoresis (1-D gel) technique in reducing condition. The first protein extraction, M1 (95% ethanol) showed obviously 15 protein bands which molecular weights were 14.97, 17.90, 18.30, 21.63, 28.54, 31, 33.96, 35.02, 42, 51.69, 65.69, 71.54, 88.02, 106.86 and 130 kDa, respectively. While M2 extraction (phenol-methanol/ammonium acetate) and M3 extraction (1.5 mM tris-HCl pH 8.0, 5 mM EDTA, 2% SDS) had low protein expression and no sharpness (13 and 12 protein bands, respectively). In different extraction conditions, therefore, M1 was a suitable method because of highest protein bands and obvious protein expression on longan pulp for proteomic analysis. Proteomic analysis of M1 extraction method was used in protein analysis by using LC-MS / MS techniques. It was found that the heat shock protein 83 (81.0 kDa), a family of proteins that was produced by cells in response to exposure on stressful conditions, the elongation factor 1-alpha (49.45 kDa), a selective regulator of translation, and the peroxidase 4 (39.74 kDa), a protein that is involved in the degeneration or aging of cells. These proteins exhibited a darker appearance of the protein bands at 30 weeks. Moreover, a partial glyceraldehyde-3-phosphate dehydrogenase (34.06 kDa), the protein involved in metabolic processes in glucose degradation, was also founded a darker appearance at 25 weeks and low appearance at 30 weeks of abnormal longan. However, higher proteomic techniques should be studied to confirm this biomarker protein in the further.
作者: Pattaranut Eakwaropas, Nakuntwalai Wisidsri
摘要: Jasminum sambac (J. sambac) has been used as a medicinal herb with wound healing property. This experimental research proposed to evaluate the effect of J. sambac flower extracts on fibroblast cell viability and film characteristics which maybe benefit in development of wound dressing film. The testing extract of J. sambac flowers were prepared as filtration extract (F), compression of the residue flower extract (C), combination of filtration and compression extract (FC) and ethanolic extract (E). The testing extracts were evaluated the effect on fibroblast cell viability using resazurin reduction assay. NIH 3T3 fibroblast cells were treated with variable concentration of J. sambac extracts for 24, 48 and 72 h. prior to evaluate cell viability. The results demonstrated that the F and FC extract of J. sambac flower at concentration of 31.25-500 µg/ml did not induce cytotoxicity on NIH 3T3 fibroblast cells given non-cytotoxic effect. The cell viability seemed to increase at 48 h of treatment with concentration of 31.25-250 µg/ml. Meanwhile, C and E extract induced cytotoxicity on NIH 3T3 cells by reduction of cell viability. The F and FC non-cytotoxic extracts were selected to examine ability of film formation without film-based compounds and assessed physical and mechanical characteristics of the prepared films. The experimental results revealed F and FC extracts could form film after incubation at 60°C. The better physical and mechanical characteristics of the films was presented in FC film which the prepared film revealed smooth surface and better absorption and elongation. Furthermore, gelatin was blended to FC extract for assess improvement of film properties. The results revealed that gelatin-blended FC extract film gave better mechanical characteristics of the film. Non-cytotoxicity of the FC extract of J. sambac flowers on fibroblast cells and appropriate physical and mechanical characteristics of FC film may point to the usefulness of J. sambac flower extracts in herb-based film for wound dressing.
作者: Suradwadee Thungmungmee, Nakuntwalai Wisidsri, Warachate Khobjai
摘要: Phenolic compounds are a large group of phytochemical compounds in plants which have antioxidant activity. Hibiscus species were used in traditional medicine in North-America with biological activities. In this study, total phenolic content, total flavonoid content and antioxidant activities of Chaba maple (Hibiscus acetosella) flower extract (CFE) in Thailand were investigated. The total phenolic and flavonoid contents were determined by Folin-Ciocalteu assay and aluminum chloride colorimetric method, respectively. Antioxidant activities of CFE were examined by DPPH radical scavenging, ferric reducing antioxidant power (FRAP) and nitric oxide (NO) radical scavenging ability methods. The results showed that total phenolic content and total flavonoid content were 91.22±0.68 mg gallic acid equivalent/g fresh matter and 0.31±0.01 mg quercetin equivalent/g fresh matter, respectively. For antioxidant activities, IC50 of DPPH radical scavenging was 57.93±0.37 μg/ml, the power of ferric reducing was 1,707.18±1.99 μM and IC50 of NO radical scavenging was 229.30±0.71 μg/ml. In conclusion, CFE could be potentially used as a new natural bioactive ingredient for nutritional supplements and cosmetics application.
作者: Nararat Thongsrinoon, Netnapha Phiwdee, Yanada Duangsa, Khaengkhae Muensub, Vichayaporn Duang-Iad
摘要: Benzoic acid analysis in curry paste samples were carried out by using high performance liquid chromatography using ultrasonic extraction. Methanol-0.05 M ammonium acetate buffer pH 4.40 in the ratio of 55:45 (%v/v) at a flow rate of 1.00 mL/min was used as the mobile phase and benzoic acid detection was performed at 226 nm using an UV-Visible detector. Under the optimum conditions, linearity of spiked samples ranged from 50 to 3,000 mg/kg. Matrix matched calibrations had determined that benzoic acid contents in southern sour, red, and green curry paste samples were 67.59, 78.62 and 72.33 mg/kg, respectively. Recoveries were obtained from 89.34 to 101.70%, 83.37 to 130.30% and 92.75 to 113.56% with R.S.D. ranged from 2.71 to 6.53%, 4.02 to 11.58% and 5.81 to 6.35%, for southern sour, red, and green curry paste samples, respectively.
作者: Techaoei Surachai, Jarmkom Khemjira, Khobjai Warachate
摘要: The main objective aimed to compare in vitro antioxidant power of different recipes of Thai herbal teas including of Tatirot, Krajeab, Kamfoi, and Kesorn Bua. The ferric reducing/antioxidant power (FRAP) assay was used to measure the total antioxidant power of freshly brewed tea. Results showed that different Thai tea recipes had slightly different in vitro antioxidant power. The herbal teas recipe was expressed as µM of antioxidant power/g of dried Thai tea recipes. Values ranges as 555.62±0.77-908.43±0.69 µM/1g of Thai herbal tea, especially Krajeab tea showed strongly antioxidant of 908.43±0.69 µM/1g of tea when compared with other samples. Therefore, it has confirmed that the antioxidant power of Thai herbal tea recipes is considerably intermediate activity than vitamin C
作者: Khemjira Jarmkom, Nakuntwalai Wisidsri, Pattaranut Eakwaropas, Warachate Khobjai
摘要: All parts of N. nucifera are used as oriental medicine for various medicinal purposes. The aimed of this study was to determine phenolic compound and antioxidant activity of different parts of three lotus (N. nucifera including of Roseum Plenum (RP), Album Plenum (AP), and Hindu Lotus (HL)). Total phenolic content using Folin-Ciocalteu reagent method and free radical scavenging activities using DPPH assay were determined. Three deferent parts of lotus (leaves, stem, and flower) were determined. The result indicated that Roseum Plenum leaf showed the highest total phenolic contents and Roseum Plenum flower showed the highest antioxidant activities. Total phenolic content of different part of different of N. nucifera showed a significant difference (p < 0.05). It also was not uniform when compare between different of N. Nucifera. The results found that the total phenolic content was the opposite of antioxidant activity. This result may be attributed to antioxidant activity may not be from phenolic content. Therefore, it is important to research and develop the potential of lotus extraction in the future.