Effects of Elevated Carbon Dioxide and Ozone on Foliar Flavonoids of Ginkgo biloba



To investigate the effect of elevated CO2 and O3 on the accumulation of flavonoids in Ginkgo Biloba leaves, four-year-old trees were exposed in open-top chambers with ambient and twice ambient CO2 and O3 concentrations singly and in combination in 2006. The results show that elevated CO2 reduce the concentrations of keampferol aglycon (-10%), isorhamnetin aglycon (-15%). Elevated O3 reduce the concentrations of the isorhamnetin aglycon (-7%), but increase the concentration of quercetin aglycon (+6%). Under elevated CO2 and O3 in combination, O3-derived effects on flavonoids concentrations are changed by elevated CO2, which are similar to that under the elevated CO2 alone. In conclusion, the concentrations of flavonoids are influenced by the changes in leaf dry mass induced by elevated CO2 and elevated O3. Furthermore, some flavonoids may not respond as antioxidant under ozone stress in ginkgo leaves.




Zhenyu Du and X.B Sun






W. Huang et al., "Effects of Elevated Carbon Dioxide and Ozone on Foliar Flavonoids of Ginkgo biloba", Advanced Materials Research, Vols. 113-116, pp. 165-169, 2010


June 2010




[1] IPCC Climate Change 2001: http: /www. ipcc. ch/pub/spm22-01. pdf.

[2] M.M. Caldwell, R. Robberecht, S.D. Flint: Internal filters: prospects for UV-acclimation in higher plants. Physiol. Plantarum Vol. 58 (1983), pp.445-450.

DOI: 10.1111/j.1399-3054.1983.tb04206.x

[3] G.A. Cooper-Driver, M. Bhattacharya: Role of phenolics in plant evolution. Phytochemistry Vol. 49 (1998), pp.1165-1174.

[4] D.S. Seigler: Plant Secondary Metabolism. Kluwer Academic Publishers, Boston(1998).

[5] X.Y. He, Y.N. Ruan, W. Chen, T. Lu: Responses of the anti-oxidative system in leaves of Ginkgo Biloba to elevated ozone concentration in an urban area. Bot. Stud. Vol. 47 (2006), pp.409-416.

[6] P.A. Peltonen, R. Julkunen-Tiitto, E. Vapaavuori, J.K. Holopainen: Effects of elevated carbon dioxide and ozone on aphid oviposition preference and birch bud exudate phenolics. Global Change Biol. Vol. 12(2006), pp.1670-1679.

DOI: 10.1111/j.1365-2486.2006.01226.x

[7] A.R. Hasler: High-performance liquid chromatographic determination of five flavonoid aglycones. J. Chromatogr. Vol. 508 (1990), pp.236-240.

DOI: 10.1016/s0021-9673(00)91262-7

[8] D.L. Haase, R. Rose: Vector analysis and its use for interpreting plant nutrient shifts in response to silvicultural treatments. Forest Sci. Vol. 41 (1995), pp.56-66.

[9] H. Saxe, D.S. Ellsworth, J. Heath: Tree and forest functioning in an enriched CO2 atmosphere. New Phytol. Vol. 139(1998), pp.395-436.

DOI: 10.1046/j.1469-8137.1998.00221.x

[10] L. Skärby, H. Ro-Poulson, F.A.M. Wellburn, L.J. Sheppard: Impacts of ozone on forests: a European perspective. New Phytol. Vol. 139(1998), pp.109-122.

DOI: 10.1046/j.1469-8137.1998.00184.x

[11] M.K. Kuokkanen, P. Niemelä, S.C. Yan: Effects of elevated CO2 and temperature on the leaf chemistry of birch Betula pendula (Roth) and the feeding behaviour of the weevil Phyllobius maculicornis. Agric. For. Entomol. Vol. 5 (2003), pp.209-217.

DOI: 10.1046/j.1461-9563.2003.00177.x

[12] A . Lavola, R. Julkunen-Tiitto: The effect of elevated carbon dioxide and fertilization on primary and secondary metabolites in birch, Betula pendula(Roth). Oecologia Vol. 99 (1994), pp.315-321.

DOI: 10.1007/bf00627744

[13] W.J. Mattson, R. Julkunen-Tiitto, D.A. Herms: CO2 Enrichment and carbon partitioning to phenolics: do plant responses accord better with the protein competition or the growth-differentiation balance models?. Oikos Vol. 111(2005), pp.337-347.

DOI: 10.1111/j.0030-1299.2005.13634.x

[14] A. Lavola: Accumulation of flavonoids and related compounds in birch induced by UV-B irradiance, Tree Physiol. Vol. 18(1998), pp.53-58.

DOI: 10.1093/treephys/18.1.53

[15] M. Kanoun, M.J.P. Goulas, J.P. Biolley: Effect of a chronic and moderate ozone pollution on the phenolic pattern of bean leaves (Phaseolus vulgaris L. cv. Nerina): relations with visible injury and biomass production. Biochem. Syst. Ecol. Vol. 29(2001).

DOI: 10.1016/s0305-1978(00)00080-6

[16] H.M. Appel: Phenolics in ecological interactions: The importance of oxidation. J. Chem. Ecol. Vol. 19(1993), pp.1521-1552.

[17] J. Gershenzon:. The cost of plant chemical defense against herbivory: a biochemical perspective. In: Insect-Plant Interactions, Vol. 5 (ed. Bernays EA), CRC Press, Boca Raton (1994), pp.105-173.

[18] P. Dizengremel: Effects of ozone on the carbon metabolism of forest trees. Plant Physiol. Bioch. Vol. 39(2001), pp.729-742.

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