Biomimetic Synthesis of the Nanocomposite of Phosphorylatd Chitosan and Hydroxyapatite and its Bioactivity In Vitro



Phosphorylated chitosan (PCS) was used as the template to control hydroxyapatite(HAp) growth, a novel nanocomposite composed of PCS and HAp was synthesized by biomimetic method. Calcium phosphate (Ca 2+= 60mM, Ca/P = 1.67) solution in HCl was added dropwise into PCS solution in NaOH. The precipitate was lyophilized to obtain the composite. The biocompatibility of the PCS-HAp nanocomopite was evaluated by osteoblast culture in vitro. The results showed that low crystallized HAp nanocrystals was formed on the PCS fibers and its crystallographic c-axis were aligned preferentially parallel to the long axis direction of PCS; the composite have good biocompatibility in vitro. It is expected that the novel composite to be a potential material for bone repair.





Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu




Q. L. Li et al., "Biomimetic Synthesis of the Nanocomposite of Phosphorylatd Chitosan and Hydroxyapatite and its Bioactivity In Vitro", Key Engineering Materials, Vols. 330-332, pp. 721-724, 2007


February 2007




[1] S.I. Stupp, P.V. Braun: Science. Vol. 277 (1997), p.1242.

[2] D Green, D Walsh, S Mann: R. O. C. Oreefo. Bone Vol. 30 (2002), p.810.

[3] S Weiner, L Addad, and H. D. Wagner: Mater Sci and Eng Vol. 11 (2000), p.1.

[4] M Sarikaya, C Tamerler, A K Y. JEN, K Schulten, F Baneyx: nature materials , Vol. 2 (2003), p.577.

[5] J.D. Hartgerink, E Beniash and S.I. Stupp: Science. Vol. 294 (2001), p.1684.

[6] Du C, Cui FZ, Zhang W, Feng QL, Zhu XD, de Groot: J Biomed Mater Res Vol. 50 (2000), p.518.

[7] M Tanahashi, T Matsuda. J Biomed Mater Res. Vol. 34 (1997), p.305.

[8] A. Tampieri, G. Celotti, E. Landi. Anal Bioanal Chem Vol. 381 (2005), p.568.

[9] Z Chen, Q-L Li, Q Zen1, G Li1, H Jiang, L Liu and B W Darvell. Key Engineering Materials Vols. 288-289 (2005) p.429.

[10] O.N. Tretinnikov, K Kato, Y Ikada. J Biomed Mater Res. 28(1994), p.1365.

[11] K Miyatakea, Y Okamotoa, Y Shigemasac, S Tokurab, S Minamia. Carbohydrate Polymers, Vol 53 (2003), p.417.

[12] Okamoto Y, Shibazaki K, Minami S, Matsuhashi A, Tanioka S, Shigemasa J Vet Med Sci, Vol 57 (1995), p.851.