Apatite Formation on α-Tricalcium Phosphate/Poly(D,L-Lactide-Co-Glycolide) Nanocomposite



In this study, a biocomposite comprising nanostructured α-tricalcium phosphate (α-TCP) in a poly(D,L-lactic-co-glycolic acid) (PLGA) matrix was fabricated by a modified solution evaporation method. As a potential temporary bone fixation and substitution material, its bioactivity was evaluated by its ability to form bone-like apatite layer in simulated body fluid (SBF). Owing to the increased surface area covered by the osteoconductive bioceramic of α-TCP, rapid apatite formation was observed. After 7 days of immersion, enhanced nucleation of apatite was observed on the nanocomposite. At day 14, dense lamellar-like apatite was formed on the nanocomposite whilst apatite nucleation had only just started to develop on the surface of pure PLGA. At the same time, a preliminary in-vitro cell culture study was conducted using human osteoblast-like (HOB) cells. A significant increase in cell number with culturing time was observed for the nanocomposite. After 9 days incubation, a confluent lamellar-like apatite layer was formed on the composite surface. This apatite layer was also shown beneath the proliferating HOB cells at Day 16.





Guy Daculsi and Pierre Layrolle




Z. Yang et al., "Apatite Formation on α-Tricalcium Phosphate/Poly(D,L-Lactide-Co-Glycolide) Nanocomposite", Key Engineering Materials, Vols. 361-363, pp. 459-462, 2008


November 2007




[1] R. Murugan and S. Ramakrishna: Compos. Sci. Technol. Vol. 65 (2005), p.2385.

[2] M.J. Karp, M.S. Shoichet and J.E. Davies: J. Biomed. Mater. Res. Vol. 64 (2003), p.388.

[3] W.L. Murphy, D.H. Kohn and D.J. Mooney: J. Biomed. Mater. Res. Vol. 50 (2000), p.50.

[4] H. Liu, E.B. Slamovich and T.J. Webster: J. Biomed. Mater. Res. Vol. 74A (2005), p.677.

[5] X. Qu, W. Cui, F. Yang, C. Min, H. Shen, J. Bei and S. Wang: Biomater. Vol. 28 (2007), p.9.

[6] Z. Yang, E.S. Thian, S.M. Best and R.E. Cameron: Key Eng. Mater. Vol. 330 (2007), p.511.

[7] T. Kokobu and H Takadama: Biomater. Vol. 27 (2006), p.2907.

[8] E.S. Thian, J. Huang, S.M. Best Z.H. Barber and W. Bonfield: J. Biomed. Mater. Res. Vol. 76B (2006), p.326.

[9] T. Kokubo, S. Ito, Z.T. Huang, T. Hayashi, S. Sakka, T. Kitsugi and T. Yamamuro: J. Biomed. Mater. Res. Vol. 24 (1990), p.331.